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host bacteria strains escherichia coli atcc 25922  (ATCC)


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    ATCC host bacteria strains escherichia coli atcc 25922
    Host Bacteria Strains Escherichia Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 49738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/host bacteria strains escherichia coli atcc 25922/product/ATCC
    Average 99 stars, based on 49738 article reviews
    host bacteria strains escherichia coli atcc 25922 - by Bioz Stars, 2026-06
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    Identification of phage-resistant bacteria: the spot test ( A ) and phage amplification-TaqMan qPCR assay ( B ). Note: Control (phage pB23 alone). W (wild-type host bacteria <t>B.m#2023).</t> R/R 1 /R 2 (phage-resistant bacteria). Δ gtr9 (gene knockout of gtr9 ). Δ gtr9::gtr9 ( gtr9 -complemented strain). Representative results of the spot test showing the presence or absence of a lytic zone after dripping 5 µL of phage suspension (10⁸ PFU/mL) onto a lawn of B.m#2023 and incubating at 37°C for 12 h. The phage amplification-qPCR assay was performed by mixing equal volumes of bacterial and phage solutions (~10⁷ CFU/mL/PFU/mL), incubating at 37°C (180 rpm, 10 min), and collecting the supernatant via centrifugation (4°C, 12,000 rpm, 5 min) as the qPCR template. Error bars represent SD of three independent experiments (*, P -value <0.05).
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    Identification of phage-resistant bacteria: the spot test ( A ) and phage amplification-TaqMan qPCR assay ( B ). Note: Control (phage pB23 alone). W (wild-type host bacteria <t>B.m#2023).</t> R/R 1 /R 2 (phage-resistant bacteria). Δ gtr9 (gene knockout of gtr9 ). Δ gtr9::gtr9 ( gtr9 -complemented strain). Representative results of the spot test showing the presence or absence of a lytic zone after dripping 5 µL of phage suspension (10⁸ PFU/mL) onto a lawn of B.m#2023 and incubating at 37°C for 12 h. The phage amplification-qPCR assay was performed by mixing equal volumes of bacterial and phage solutions (~10⁷ CFU/mL/PFU/mL), incubating at 37°C (180 rpm, 10 min), and collecting the supernatant via centrifugation (4°C, 12,000 rpm, 5 min) as the qPCR template. Error bars represent SD of three independent experiments (*, P -value <0.05).
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    Identification of phage-resistant bacteria: the spot test ( A ) and phage amplification-TaqMan qPCR assay ( B ). Note: Control (phage pB23 alone). W (wild-type host bacteria B.m#2023). R/R 1 /R 2 (phage-resistant bacteria). Δ gtr9 (gene knockout of gtr9 ). Δ gtr9::gtr9 ( gtr9 -complemented strain). Representative results of the spot test showing the presence or absence of a lytic zone after dripping 5 µL of phage suspension (10⁸ PFU/mL) onto a lawn of B.m#2023 and incubating at 37°C for 12 h. The phage amplification-qPCR assay was performed by mixing equal volumes of bacterial and phage solutions (~10⁷ CFU/mL/PFU/mL), incubating at 37°C (180 rpm, 10 min), and collecting the supernatant via centrifugation (4°C, 12,000 rpm, 5 min) as the qPCR template. Error bars represent SD of three independent experiments (*, P -value <0.05).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Gtr9 mutation trades phage resistance for carbapenem sensitivity to potentiate phage-meropenem therapy against carbapenem-resistant Acinetobacter baumannii in vitro

    doi: 10.1128/aac.01355-25

    Figure Lengend Snippet: Identification of phage-resistant bacteria: the spot test ( A ) and phage amplification-TaqMan qPCR assay ( B ). Note: Control (phage pB23 alone). W (wild-type host bacteria B.m#2023). R/R 1 /R 2 (phage-resistant bacteria). Δ gtr9 (gene knockout of gtr9 ). Δ gtr9::gtr9 ( gtr9 -complemented strain). Representative results of the spot test showing the presence or absence of a lytic zone after dripping 5 µL of phage suspension (10⁸ PFU/mL) onto a lawn of B.m#2023 and incubating at 37°C for 12 h. The phage amplification-qPCR assay was performed by mixing equal volumes of bacterial and phage solutions (~10⁷ CFU/mL/PFU/mL), incubating at 37°C (180 rpm, 10 min), and collecting the supernatant via centrifugation (4°C, 12,000 rpm, 5 min) as the qPCR template. Error bars represent SD of three independent experiments (*, P -value <0.05).

    Article Snippet: Whole-genome sequences of three phage-resistant mutants (R, R 1 , R 2 ) and wild host bacteria B.m#2023 were mapped to the reference genome ATCC 19606 ( GCA_009035845.1 ), respectively.

    Techniques: Bacteria, Spot Test, Amplification, Control, Gene Knockout, Suspension, Centrifugation

    Phenotype analysis of W, R, Δ gtr9, and Δ gtr9::gtr9 . ( A ) Bacterial growth curve. ( B ) Assessment of biofilm formation based on viable bacterial counts. ( C ) Capsule production was measured by uronic acid content, and normalization was performed using the W results as the reference. ( D–G ) Transmission electron micrograph. Note: W (wild-type host bacteria B.m#2023), R (phage-resistant bacteria), Δ gtr9 (gene knockout of gtr9 ), Δ gtr9::gtr9 ( gtr9 -complemented strain). Error bars represent SD of three independent experiments (*, P -value <0.05).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Gtr9 mutation trades phage resistance for carbapenem sensitivity to potentiate phage-meropenem therapy against carbapenem-resistant Acinetobacter baumannii in vitro

    doi: 10.1128/aac.01355-25

    Figure Lengend Snippet: Phenotype analysis of W, R, Δ gtr9, and Δ gtr9::gtr9 . ( A ) Bacterial growth curve. ( B ) Assessment of biofilm formation based on viable bacterial counts. ( C ) Capsule production was measured by uronic acid content, and normalization was performed using the W results as the reference. ( D–G ) Transmission electron micrograph. Note: W (wild-type host bacteria B.m#2023), R (phage-resistant bacteria), Δ gtr9 (gene knockout of gtr9 ), Δ gtr9::gtr9 ( gtr9 -complemented strain). Error bars represent SD of three independent experiments (*, P -value <0.05).

    Article Snippet: Whole-genome sequences of three phage-resistant mutants (R, R 1 , R 2 ) and wild host bacteria B.m#2023 were mapped to the reference genome ATCC 19606 ( GCA_009035845.1 ), respectively.

    Techniques: Transmission Assay, Bacteria, Gene Knockout

    The survival curve of the zebrafish after inoculation with PBS buffer or different concentrations ( A : 5 × 10 4 and B : 5 × 10 7 CFU/mL) of Acinetobacter baumannii solution. Note: W (wild-type host bacteria B.m#2023), R (phage-resistant bacteria), Δ gtr9 (gene knockout of gtr9 ), Δ gtr9::gtr9 ( gtr9 -complemented strain).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Gtr9 mutation trades phage resistance for carbapenem sensitivity to potentiate phage-meropenem therapy against carbapenem-resistant Acinetobacter baumannii in vitro

    doi: 10.1128/aac.01355-25

    Figure Lengend Snippet: The survival curve of the zebrafish after inoculation with PBS buffer or different concentrations ( A : 5 × 10 4 and B : 5 × 10 7 CFU/mL) of Acinetobacter baumannii solution. Note: W (wild-type host bacteria B.m#2023), R (phage-resistant bacteria), Δ gtr9 (gene knockout of gtr9 ), Δ gtr9::gtr9 ( gtr9 -complemented strain).

    Article Snippet: Whole-genome sequences of three phage-resistant mutants (R, R 1 , R 2 ) and wild host bacteria B.m#2023 were mapped to the reference genome ATCC 19606 ( GCA_009035845.1 ), respectively.

    Techniques: Bacteria, Gene Knockout